PCR Technology: Current Innovations, Second EditionThomas Weissensteiner, Tania Nolan, Stephen A. Bustin, Hugh G. Griffin, Annette Griffin CRC Press, 2003. nov. 13. - 416 oldal A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of |
Tartalomjegyzék
Chapter 3 Quantitative mRNA Analysis in Small Cell Samples by RTPCR and Flow Cytometry | 23 |
Chapter 4 Microdissection of Single or Small Numbers of Cells for Analyses of DNA and RNA by PCR | 29 |
Chapter 5 PrePCR Processing Strategies | 37 |
Chapter 6 Novel PCREnhancing Compounds and Their Modes of Action | 51 |
Chapter 7 Optimizing PCR with the Aid of Experimental Design | 65 |
Chapter 8 Economic Fluorescent Labeling of PCR Products for Microsatellite and Single Stranded Conformation Polymorphism SSCP Analysis | 79 |
Chapter 9 Universal Restriction SiteFree Cloning Method Using Chimeric Primers | 87 |
Analytical Applications | 93 |
Chapter 21 Detection of a SingleCopy Sequence on Human Metaphase by Cyclic PRINS | 215 |
Chapter 22 Meaningful Quantification of mRNA Using RealTime PCR | 225 |
Chapter 23 Absolute Quantification of Specific Nucleic Acids by RTPCR Using a Nonlinear Mathematical Model for Data Analysis | 235 |
Chapter 24 SingleMolecule PCR Basic Protocols and Applications | 245 |
Application of a GenomeWide Approach | 259 |
Chapter 26 The Detection of Differential Gene Expression Changes Using SAGE Serial Analysis of Gene Expression | 267 |
Chapter 27 Applications of Differential Display in Infectious Diseases | 277 |
READS | 287 |
Chapter 10 HighThroughput Genotyping and the PCR | 97 |
Chapter 11 HighThroughput Genetic Analysis through Multiplexed PCR and Multicapillary Electrophoresis | 111 |
A PurificationFree Assay for Genotyping by MALDI Mass Spectrometry | 121 |
Chapter 13 Primer Design for LargeScale Multiplex PCR and Arrayed Primer Extension APEX | 131 |
Chapter 14 Surface Plasmon Resonance Based Biosensor Technology for RealTime Detection of PCR Products | 141 |
Chapter 15 Fluorescent Amplified Fragment Length Polymorphism FAFLP Genotyping | 155 |
Chapter 16 Global Analysis of DNA Allelic Variations GADAV by Specific Enrichment of Mismatches and Selective Amplification of Heterohybrids | 163 |
Chapter 17 QuantitativeDNAMethylation Analysis by the Bisulfite Conversion Method | 175 |
Chapter 18 Pyrosequencing Technology | 187 |
Chapter 19 OctamerPrimed Cycle Sequencing | 197 |
Chapter 20 Amplification of cDNA | 205 |
Chapter 29 Suppression Subtractive Hybridization PCR | 299 |
Preparative Application | 309 |
Chapter 30 PCRBased Techniques for Cloning an Unknown DNA Fragment Adjacent to a Known Integrant | 313 |
Chapter 31 Chromosome Walking by Inverse PCR | 323 |
Chapter 32 Long Universal Fast Walking | 329 |
Chapter 33 Cloning of Unknown Virus Sequences by DNase Treatment and Sequence Independent Single Primer Amplification | 337 |
Chapter 34 Whole Genome Amplification from Single Cells and Minute DNA Samples | 349 |
Efficiency and Bias | 361 |
Chapter 36 Evolution through PCRMediated Mutagenesis and Recombination | 371 |
Back cover | 381 |
Más kiadások - Összes megtekintése
PCR Technology: Current Innovations, Second Edition Thomas Weissensteiner,Tania Nolan,Stephen A. Bustin,Hugh G. Griffin,Annette Griffin Nincs elérhető előnézet - 2003 |
Gyakori szavak és kifejezések
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