Vascular Effects of Hydrogen Sulfide: Methods and ProtocolsJerzy Bełtowski Springer Nature, 2019. máj. 30. - 221 oldal This book explores techniques for exploring hydrogen sulfide (H2S) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular H2S generation/signaling may be involved in the pathogenesis of systemic and pulmonary arterial hypertension, ischemic heart disease, ischemic stroke, preeclampsia, and erectile dysfunction, and H2S also serves as an attractive target for pharmacotherapy of cardiovascular diseases, as well as possible effects on cancer, wound healing, and diabetic retinopathy, among other pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Vascular Effects of Hydrogen Sulfide: Methods and Protocols is an ideal aid for scientists working to extend our knowledge in this valuable and wide-ranging field of study. |
Tartalomjegyzék
| 1 | |
| 9 | |
Chapter 3 Analysis of Vascular Hydrogen Sulfide Biosynthesis | 19 |
Chapter 4 Measurement of Protein Persulfidation Improved TagSwitch Method | 37 |
Chapter 5 ProPerDP A Protein Persulfide Detection Protocol | 51 |
Chapter 6 Vascular Effects of H2SDonors Fluorimetric Detection of H2S Generation and Ion Channel Activation in Human Aortic | 79 |
Chapter 7 In Vitro Measurement of H2SMediated Vasoactive Responses | 89 |
Chapter 8 In Vivo Measurement of H2S Polysulfides and SSNO Mix ́ ́Mediated Vasoactive Responses and Evaluation of Ten Hem | 109 |
Chapter 10 The Relaxant Mechanisms of Hydrogen Sulfide in Corpus Cavernosum | 137 |
Chapter 11 Pharmacological Tools for the Study of H2S Contribution to Angiogenesis | 151 |
Chapter 12 Central Administration of H2S Donors for Studying Cardiovascular Effects of H2S in Rats | 167 |
Chapter 13 Colonic Delivery of H2S Donors for Studying Cardiovascular Effects of H2S in Rats | 173 |
Chapter 14 Measurements for SulfideMediated Inhibition of Myeloperoxidase Activity | 179 |
Chapter 15 Vascular Myography to Examine Functional Responses of Isolated Blood Vessels | 205 |
| 219 | |
Chapter 9 Simultaneous Measurements of Tension and Free H2S in Mesenteric Arteries | 125 |
Más kiadások - Összes megtekintése
Vascular Effects of Hydrogen Sulfide: Methods and Protocols Jerzy Bełtowski Nincs elérhető előnézet - 2020 |
Vascular Effects of Hydrogen Sulfide: Methods and Protocols Jerzy Bełtowski Nincs elérhető előnézet - 2019 |
Gyakori szavak és kifejezések
acid activity alkylated angiogenesis antibody aorta assay beads Biol blood buffer cannula cardiovascular carotid catheter cavernosal CBS and CSE channels Chem compounds corpus cavernosum curve cystathionine cysteine detection dilution disulfide endogenous H2S endothelial cells endothelium enzyme erectile dysfunction exogenous experiment filter final concentration fluorescence function gasotransmitter gelatin GSNO H2S donor H2S-donor H2S-induced human hydrogen sulfide Incubate infusions inhibition inhibitor Kimura H Krebs solution measurement mechanisms membrane mesenteric artery method mouse myeloperoxidase myograph Na2S NaCl Nagy NaHS neutrophil nitric oxide Note organ bath pathway penile peroxidase Pharmacol Physiol physiological polysulfide Prepare ProPerDP protein protein persulfides protocol reaction reactivity reagent Redox Signal relaxation response role samples sensor serum smooth muscle species Springer Science+Business Media staining step sterile stock solution sulfur supernatant tag-switch Thermo Fisher Scientific thiols tion tube ultrapure water Vascular Effects vasoactive vasorelaxation vessel vitro Wash μΜ